An extension to: Systematic assessment of commercially available low-input miRNA library preparation kits

We compared the performance of two commercial kits (NEBNext and NEXTflex) for miRNA detection sensitivity, reliability, titration response and ability to detect differentially expressed miRNAs at different amounts of synthetic miRNAs. We observed differences in detection sensitivity between the kits, but none were able to detect the full repertoire of expected miRNAs. The reliability within the replicates of all kits was good, while larger differences were observed between the kits, although none could accurately quantify the majority of miRNAs. In a previous publication (https://doi.org/10.1080/15476286.2019.1667741; GEO accession number GSE133719) we accessed the performance of six additional miRNA library preparation kits. Systematic comparison of miRNA profiles assessed in low-input synthetic miRNA samples and samples from pools of blood derived CD8 T cells of rheumatoid arthritis (RA) patients or healthy controls (HC) by six commercially available library preparation kits. In a previous publication (https://doi.org/10.1080/15476286.2019.1667741; GEO accession number GSE133719) we accessed the performance of six additional miRNA library preparation kits.