Mapping the Spatial Proteome of Leukemia Cells Undergoing Fludarabine Treatment

Recent advancements in high-throughput ex vivo drug profiling have spurred interest in functional precision medicine, aiming to personalize cancer treatment based on an individual's cellular characteristics. This proof-of-concept study utilizes Molecular Pixelation (MPX), a single-cell spatial proteomics platform, for quantifying protein abundance, spatial distribution, and co-localization of targeted proteins in leukemia cells. Using the glucocorticoid-resistant ETV6/RUNX1 acute lymphoblastic leukemia cell-line Reh, we investigated fludarabine, a drug used prior to allogenic stem cell transplantation and chimeric antigen receptor T-cell therapy.