Bacteroides thetaiotaomicron ameliorates colon inflammation in preclinical models of Crohn’s Disease [mouse colon]

Analysis of the gut transcriptome as affected by treatment with Bacteroides thetaiotaomicron in a mouse model of IBD Here, we report analysis of the gut transcriptome in IL10KO mice after treatment with Bacteroides thetaiotaomicron (BT). Comparative transcriptome analysis of ascending colon tissue of IL10KO and IL10KO/BT-treated mice showed differential expression of a wide range of genes associated with inflammatory responses between the two treatment groups. Expression of proinflammatory genes and genes involved in pathogen recognition was particularly lower in IL10KO/BT mice. IL10KO mice were dosed with BT 3 times a week from weaning at 3 weeks old, for a total period of 13-14 weeks. Control IL10KO mice were dosed with culture medium alone.