Differentially expressed (DE) genes after the injection of alx1 mRNA into starfish.

(A) MAplot of DE genes identified by RNAseq. Data represent individual putative gene in a log2 ratio versus log2 average plot. Red plots indicate DE genes with q value (FDR) ApEF1a. Data from three trials using different batches of eggs are shown. Black points indicate experimental data derived using sea urchin Hpalx1, and gray points show data derived using starfish Apalx1. The horizontal bars indicate mean values. Asterisks indicate significant differences between ΔCt values of sample pairs (paired t-test with Bonferroni correction, p