Coordination polymers constructed from 5-nitro-1,2,3-benzenetricarboxylic acid: crystal structures and treatment effect on nephrotic syndrome by regulating intestinal flora and recovering Th17/Treg balances

Two new transition metal-based coordination polymers [Zn3(bimb)2(nbta)2]n (1, bimb = 1,4-bis(imidazol-1-yl)-2-butene, H3nbta = 5-nitro-1,2,3-benzenetricarboxylic acid) and {[Cu3(nbta)2(bipp)2(H2O)2](H2O)2}n (2, bipp = 1,4-bis(pyridin-4-ylmethyl)piperazine) have been successfully prepared by reaction of 5-nitro-1,2,3-benzenetricarboxylic acid with the corresponding metal salts and the different N-donor ligands. Furthermore, the treatment effect of compounds 1 and 2 on nephrotic syndrome (NS) was evaluated and the specific mechanism was explored in this research. Firstly, to investigate the level of the Th17 cells and CD4 + CD25 + Foxp3+ regulate cells (Treg) in PBMC was detected with flow cytometry. The RT-PCR was performed to analyze the mRNA expression level of 16sRNA to determine the intestinal flora abundance.