Single cell RNA sequencing of myeloid cells from lungs of young and aged mice. Single cell RNA sequencing of myeloid cells from lungs of young and aged mice

Single cell RNA sequencing from lungs of young (2-3 month old) and aged (20-22 month old) mice. Overall design: Myeloid cells (CD45+ Gr1+ CD11b+) were sorted from lungs of young and aged mice that were perfused. Cells from young mice (2-3 months old) were labeled with TotalSeq-C0951 SA antibody (Barcode sequence AACCTTTGCCACTGC), and cells from aged mice (20-22 months old) were labeled with TotalSeq-C0952 SA antibody (Barcode sequence GTCCGACTAATAGCT). Cells were then pooled for scRNAseq preparation.