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ROS activates NETs release of bone marrow CD55+ neutrophils to promote early bone aging

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Bone marrow neutrophils are highly heterogeneous cells and we used sing cell RNA-sequencing to analyze the diversity of BM neutrophils of SAMP6 and SAMR1 mice femur bone. Femour of 3-month male SAMP6 and SAMR1 mice were separately flushed out, and after lysing red cells, BM cells were analyzed by sc-RNA seq.

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