Role of KLF6 in shaping macrophage inflammatory response

Kruppel-like transcription factor 6 (KLF6) is a member of zinc-finger family transcription factors that regulate critical cellular processes including development, differentiation, proliferation, and programmed cell death. Alterations in KLF6 expression or function has been associated with the pathogenesis of numerous human ailments, including inflammatory bowel diseases, cancer, hepatic steatosis, and hepatic fibrosis. Overall design: Here we isolated bone marrow of 3 mice per group (Lyz2cre and Lyz2cre:KLF6Fl/Fl) and differentiated into macrophages by culturing them in M-CSF containing media. These cells were stimulated with Heat-killed Candida albicans (HKCA) or 50 micrograms/ml Scleroglucan for 5 hours. Total RNA was isolated and subjected to RNAseq analyses.