Full-factorial breeding experiment with lake char (Lake Geneva, winter 2017/2018)

We sampled 16 wild lake char (Salvelinus umbla) and used their gametes to investigate the genetic consequences of different mating scenarios. A full-factorial breeding was used to separate additive genetic from maternal environmental effects, and embryos were raised singly after sublethal exposures to a pathogen, a common pollutant, or water only. In all treatment groups, embryo development was strongly reduced with increased genetic relatedness between the parents. Contrary to predictions of ‘good genes’ sexual selection, pathogen tolerance of offspring declined with increasing coloration of their fathers. Determination of inbreeding and kinship coefficients: All 10 males and 4 of the 6 females were used in a parallel study that included sperm competition experiments (Nusbaumer et a. 2021). These 14 parents had been prepared in one library for ddRAD sequencing on 2 lanes on an Illumina HIseq 2500 (see de Guttry et al. 2022 for the corresponding 14 gzipped .fastq of these individuals). ...本研究采集了16尾野生湖红点鲑（Salvelinus umbla），并利用其生殖细胞开展实验，以探究不同交配情境下的遗传效应。本研究采用全因子育种设计，以区分加性遗传效应与母体环境效应；将胚胎分别置于亚致死剂量的病原体、常见污染物或仅清水（对照组）中进行暴露处理后，实施单养培育。在所有处理组中，胚胎发育均随亲本间遗传亲缘系数升高而显著受到抑制。与“优秀基因”性选择理论的预测相悖，子代的病原体耐受能力随其父体色加深而降低。 近交系数与亲缘系数的测定：本研究的10尾雄性亲鱼与6尾雌性亲鱼中的4尾，曾参与一项包含精子竞争实验的平行研究（Nusbaumer et al. 2021）。这14尾亲鱼已被构建为一个测序文库，通过Illumina HiSeq 2500平台的2个泳道进行ddRAD测序（相关个体的14个压缩.fastq文件详见de Guttry et al. 2022年的研究）。