five

Ribodepleted RNA-seq libraries from planarian tissues

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NIAID Data Ecosystem2026-05-02 收录
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https://www.ncbi.nlm.nih.gov/sra/SRP518749
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In this study, we customized and optimized an RNaseH-based ribodepletion strategy and applied it to total RNA extracted from whole planarian worms, the outer epithelium (a.k.a., epidermis), and isolated blastema tissues. Our objectives were to create comprehensive RNA-seq libraries that: 1) contain less than 1% of rRNA contamination, and 2) include transcripts that are not polyadenylated, such as those encoding histone proteins and many noncoding RNAs.
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2025-08-23
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