Post-transcriptional regulation of necroptosis gene EIF2AK2 in peripheral blood of bronchopulmonary dysplasia

Bronchopulmonary dysplasia (BPD) remains the most frequent chronic lung disease among infants, which involves multifactorial pathogenesis. Necroptosis represents a caspase-independent mode of programmed cell death, and its deregulation associates with lung diseases, but the mechanisms of necroptosis during BPD are indistinct. This work was conducted for unveiling the post-transcriptional regulatory mechanisms of necroptosis in BPD. We sequenced messenger RNA (mRNA) (BPD, n=4; controls, n=4) and microRNA (miRNA) (BPD, n=5; controls, n=5) profiles in peripheral blood specimens. The GSE125873 dataset composed of mRNA profiling from 11 BPD individuals and 21 controls was gathered. Based upon the circBank and starBase databases, circRNA-miRNA-mRNA networks and a necroptosis-based subnetwork were built. The diagnostic efficacy of eukaryotic translation initiation factor 2 alpha kinase 2 (EIF2AK2) was assessed via receiver operator characteristic curve (ROC). EIF2AK2-relevant molecules were analyzed with weighted correlation network analysis (WGCNA), and then molecular classification was conducted through consensus clustering method.