Single-cell RNAseq of Normoxic and OIR mouse retina by Drop-seq

Proliferative retinopathy is associated with abnormal vascular development (neovascularisation) of the retina. In mouse, the oxygen-induced retinopathy (OIR) model mimics the proliferative retinopathy found in extreme premature babies (Retinopathy of Prematurity) and in patients suffering from diabetic retinopathy. Using Drop-seq, we performed single-cell RNAseq analysis of mouse retina under physiological, normoxic (NORM) condition and retina under OIR condition, at postnatal day 14 and day 17, periods associated with a peak of neovascularisation. We used both whole retina cells or rod (CD73) depleted retina cells. Sorting conditions (whole vs. rod-depleted) derived count matrices were aligned with Seurat CCA and processed for dimensionality reduction and clustering. We analysed the effect of OIR/NORM by single-cell RNAseq (Drop-seq) of C57B6 retina (whole retina n =7, and rod-depleted n=5) from P14 and P17 animals. Contributor: Vision Health Research Network