Suppression of TGFβ by anti-TGFβ antibody 1D11 increased the mineral-to-collagen ratio.

Confocal raman spectroscopy was performed on mice bearing MDA-MB-231 tumors in bone treated for 4 weeks with 1D11 or 13C4 antibodies as described in materials and methods section. At least nine spectra were analyzed per specimen and the mean mineral-to-collagen ratio, Type-B carbonate substitution, and crystallinity were scored. Both mineral-to-collagen ratio and carbonate substitution increased significantly upon 1D11 treatments compared to control. Mean ± standard deviation is shown, p value was determined using Wilcoxon test.