SAIMOS - Biological and Flow Cytometry data collected from CTD stations in South Australia, in March 2019

Flow cytometry data was collected in March 2019, in waters off South Australia.

The general purpose of the study is to be able to establish background knowledge on the ecosystem on the continental shelf of South Australia and the impact of upwelling/saline outflow events on microbial communities to ultimately develop a biogeochemical model of the region. Sampling was carried out during cruises conducted on board the RV Ngerin as part of the Southern Australian Integrated Marine System (SAIMOS). During each cruise, the physical, chemical and biological properties of the chlorophyll fluorescence maximum (DCM) layer were investigated. Flow cytometry data has been collected for picophytoplankton, bacteria and viruses.

Six main stations have been sampled over the course of the study, five are located on the 100 m isobath, i.e. RS (35.508S, 136.278E), B2 (35.418S, 136.148E), B3 (35.258S, 136.048E), SAM2CP/B4 (35.168S, 135.418E) and SAM5CB/B5 (35.008S, 135.198E), and one from an offshore station (B1; 36.188S, 136.178E) located southwest of Kangaroo Island. Note that combining the distances between stations (14–25 nautical miles), the average component of the current velocity at middepth along the shelf (0.01 m s21) and the average speed of the vessel (i.e. 9 knots) indicate that different water masses were sampled at each station. Additional samples have on occasion been collected from the National Reference Station (NRS) at Kangaroo Island (35.832S, 136.447E) and the SA Spencer Gulf Mouth Mooring (SAM8SG, 35.25S, 136.690E), where the saline outflow occurs.

本数据集的流式细胞术（Flow Cytometry）数据采集于2019年3月，采集区域为澳大利亚南部近海海域。

本研究的核心目标为构建澳大利亚南部大陆架海域生态系统的背景知识体系，解析上升流/盐度流出事件对微生物群落的影响，最终建立该区域的生物地球化学模型。采样工作依托NGERIN号研究船（RV Ngerin）开展，作为南澳大利亚综合海洋系统（SAIMOS）的项目组成部分。在每一次科考航次中，研究人员均对叶绿素荧光最大值层（Deep Chlorophyll Maximum, DCM）的物理、化学及生物学特性开展调查。本数据集针对超微型浮游植物、细菌及病毒采集了流式细胞术相关数据。

本研究共布设6个主要采样站位：其中5个站位位于100米等深线处，分别为RS（35.508°S，136.278°E）、B2（35.418°S，136.148°E）、B3（35.258°S，136.048°E）、SAM2CP/B4（35.168°S，135.418°E）以及SAM5CB/B5（35.008°S，135.198°E）；剩余1个站位为位于袋鼠岛西南侧的外海站位B1（36.188°S，136.178°E）。注：结合站位间距（14~25海里）、陆架中层海流的平均流速分量（0.01 m·s⁻¹）以及研究船的平均航速（约9节），可判定各站位所采集的水样分属不同水团。研究团队还偶尔在袋鼠岛国家参考站位（NRS，35.832°S，136.447°E）以及发生盐度流出事件的斯宾塞湾口锚定站位（SAM8SG，35.25°S，136.690°E）采集补充样本。