Demonstrating voxel-by-voxel (v×v) single-point calibration in liver tissue by IR-MALDESI quantitative MSI

The quantification of glutathione (GSH) on a per-voxel basis was achieved
by homogenously spraying a known amount of stable isotope labeled
glutathione (SIL-GSH) on a microscope slide. Next, liver sections were
mounted on top of the coated slides and imaged using IR-MALDESI MSI.
Statistical analysis has shown high precision for V×V quantification over
a wide concentration range, but the accuracy of this method is currently
limited due to the sprayer configuration. Results support the feasibility
of V×V quantification as evidence by concentration heatmaps. Additionally,
voxel-based quantification provides benefits such as high specificity,
straightforward sample preparation, and promising initial statistics
analysis which will be advantageous for future qMSI studies.