Transcription profiling of mouse early skin development

Mice with the spontaneous uncv mutation are hairless. We utilize the uncv null mouse to investigate the underlying mechanisms giving rise to the relevant phenotypes and reveal significant changes in certain signaling pathways by microarray. Experiment Overall Design: Dorsal skins were taken from 4-day-old uncv null mice and wild type mice (as control), and they are sisters-german. The parent is uncv heterozygosis(uncv +/-), and their children has 3 genotype: wild(uncv +/+), heterozygosis(uncv +/-) and null (uncv -/-). We can easily distinguish them by their beard length and shape or identify their genotype by PCR using specific primers. HR 02 is a wild mouse sample and uncv null mice expresses as HR 03 and HR 04. RNA isolation was performed with the QIAGEN RNeasy mini kit (QIAGEN, Valencia, CA, USA) following standard instructions.携带自发uncv突变的小鼠表现为无毛表型。本研究采用uncv基因敲除（null）小鼠探究相关表型的潜在分子机制，并通过基因芯片（microarray）分析揭示了部分信号通路的显著变化。实验整体设计：实验取材于4日龄的uncv基因敲除小鼠与野生型小鼠（作为对照），二者为同父同母的同胞个体。其亲本为uncv杂合子（uncv +/-），子代存在三种基因型：野生型（uncv +/+）、杂合型（uncv +/-）与纯合敲除型（uncv -/-）。可通过胡须的长度与形态轻松区分各组小鼠，或使用特异性引物通过聚合酶链式反应（PCR）鉴定其基因型。HR 02为野生型小鼠样本，uncv基因敲除小鼠样本则对应HR 03与HR 04。总RNA提取按照标准操作流程，使用QIAGEN RNeasy迷你试剂盒（QIAGEN，美国加利福尼亚州巴伦西亚）完成。