Supplementary Information for "High-Throughput Profiling of Prenylelongases Enables the Assembly of Modified Prenoids"

This is the external Supplementary Information for our publication "High-Throughput Profiling of Prenylelongases Enables the Assembly of Modified Prenoids", freely available as a preprint from ChemRxiv (https://doi.org/10.26434/chemrxiv-2025-nl5ds). The .zip files contain the raw data and metadata for all items (supplementary and main text) as well as the calculation results underlying each figure and table. This includes UV, MS, and NMR data. The zip files below also contain the ChimeraX session used to prepare all figure panels. This work was enabled by our previous studies on continuous reaction monitoring of phosphate-releasing transformations (https://doi.org/10.1021/acs.analchem.1c05356 & https://doi.org/10.1002/anie.202412597), which itself builds on principles of spectral unmixing (https://doi.org/10.1002/cbic.202000204), isosbestic normalization (https://doi.org/10.1002/cbic.202200744) and thermodynamic reaction control in enzymatic equilibrium systems (https://doi.org/10.1021/acscatal.1c02589 & https://doi.org/10.1002/anie.202218492 & https://doi.org/10.1002/adsc.201901230).