Host Bacteriome Analysis Provides New Insights into the Plant Growth-Promoting Mechanisms of Pseudomonas oryzihabitans GDW1

Tomato (Solanum lycopersicum) is an important agricultural crop worldwide, including China, which contributes over 36% of the world's total tomato production. However, its cultivation is affected by various biotic and abiotic stresses, which negatively impact production and quality. Using plant growth-promoting (PGP) bacteria to improve tomato growth under these circumstances is gaining much attention as a sustainable agriculture approach. This study assessed the PGP potential of an endophytic bacterium, Pseudomonas oryzihabitans GDW1, via a multi-omic approach. . Analysis of tomato root bacteriome through 16S rRNA gene amplicon sequencing showed that GDW1 inoculation dramatically changed the bacterial community structure, enhancing diversity and abundance of advantageous microbial taxa (Proteobacteria and Bacteroidota). Co-occurrence network analysis indicated that microbial relationships grew increasingly intricate in treated plants, facilitating improved nutrient absorption and stress resilience. Further, FAPROTAX and PICRUSt2 functional prediction analysis revealed the beneficial role of GDW1 in nitrogen cycling, organic matter degradation, plant growth promotion, and plant resilience against stresses.