Osmotic stress and Hog1 induce the activity of UASru.

Cells were cultured in either SD or SA with or without 1M NaCl, or following exposure to 0.4 M NaCl and assayed for β-galactosidase activity. The results represent the average of data for three independent transformants. Strains used were diploid wt (Y1721) and its isogenic hog1Δ/hog1Δ (Y1908) and kss1Δ/kss1Δ (Y1909) strains, and a haploid wt (Y1214). All strains carried UASru-lacZ integrated within genomic LEU2. NT – Not Tested.