Dataset for PCB Sulfates in Human Serum from Mothers and Children in Urban and Rural U.S. Communities

This dataset from the Iowa Superfund Research Program (isrp) includes PCB sulfate concentrations from serum samples collected from individuals living in Columbus Junction (CJ), Iowa (N=24) and East Chicago (EC), Indiana (N=24). PCB sulfates in serum samples were extracted and then enzymatically hydrolyzed to the corresponding OH-PCBs with Helix pomatia sulfatase that had been highly purified by affinity chromatography. The resulting OH-PCBs were converted to their corresponding methoxy-PCB (MeO-PCB) derivatives which were analyzed by GC-MS/MS. Comparison of results of incubations with and without sulfatase-catalyzed hydrolysis yielded the concentration of PCB sulfates in the original sample of serum. The maximum number of PCB sulfate congeners quantitated was limited to the 74 MeO-PCB standards available. The total concentration of these PCB sulfates determined in individuals within the CJ group ranged from 530 to 6700 pg/g of serum, while the total of these PCB sulfates determined in individuals within the EC group ranged from 110 to 8900 pg/g of serum.