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Generation of ventralized thalamic organoids with inhibitory thalamic reticular nucleus

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https://www.ncbi.nlm.nih.gov/sra/SRP390431
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Single-cell RNA-seq was performed in SHH-treated thalamic organoids Overall design: Human thalamic organoids (ThOs) resembling the dorsal thalamus by dual SMAD inhibition and insulin-driven caudalization coupled with thalamic patterning (MEK-ERK inhibition and BMP7 activation) from hESCs. The organoids were further guided into ventral identity with SHH treatment.
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2023-04-25
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