XIST modulates mammary stem cell homeostasis and tumor fate [Cut&Run]

Disruption of XIST expression in mammary epithelial cells leads to chromatin changes on the inactive X chromosome, transcriptional reactivation of X-linked genes, and defective luminal differentiation. Overall design: Cells from both luminal (ML) and mammary stem cell (MaSC) compartments were isolated from wild-type and XIST-KO HMLE (Human Mammary Epithelial cells) by FACS sorting based on their positivity for the cell surface antigen EpCAM (ESA) (ML: ESA+; MaSC: ESA-). Two replicates were generated per compartment and genotype. Cells were processed for Cut&Run to map the genome-wide distribution of a panel of histone modifications.