Integrated of MicroRNA–mRNA Analysis of sterile DH Japanese flounder. Paralichthys olivaceus

Many infertility cases have an underlying genetic basis, only few genes have so far been associated with infertility. Discovering and validating new candidate genes through the study of sterile animal models by sequencing technique is important. In the present study, sterile and fertile doubled-haploid (DH) Japanese flounders at the age of 5 years as animal models were sequenced using high-throughput sequencing technology to study molecular basis of reproductive disorders.