S. cerevisiae cells: control vs positive supercoiling accumulation in absence of telomere silencing after 0 and 120 min

Positive DNA helical stress accumulates in vivo by the unbalanced relaxation of positive and negative DNA supercoils in _top1, top2ts, pGPD:TopA yeast cells在top1、top2ts、pGPD:TopA酵母细胞中，由于正负DNA超螺旋发生不平衡松弛，体内会积累DNA正向螺旋应力。