Essential role of Arid1a in intestinal stem cell maintenance and homeostasis through Sox9 regulation (ChIP-Seq)

Arid1a, a subunit of the SWI/SNF chromatin remodeling complex, have been reported in multiple human cancers, however, its functional role in intestinal homeostasis remains unclear. To investigate the role of Arid1a in murine intestine, we have employed ChIP experiments in intestinal spheroid cells generated from crypt cells of wild type mice. ChIP assay revealed that Arid1a binds directly to the Sox9 promoter to support its expression. We used intestinal spheroid cells generated from crypt cells of wild type mice.