Spatiotemporally controlled generation of NTPs for single-molecule studies of NTP-driven processes

Data supporting the findings of the paper titled "Spatiotemporally controlled generation of NTPs for single-molecule studies" in Nature Chemical Biology which describes a method for controlling NTP-driven reactions in single-molecule experiments via the local generation of NTPs (LAGOON) that increases the measurement throughput and enables single-turnover observations. 
The dataset contains single-molecule FRET data capturing T4 helicase DNA unwinding, nucleosome remodeling by Chd1 and the invasion of a nucleosome by the T7 RNA polymerase. Additionally, the dataset contains acoustic force spectroscopy data on E. coli RNA polymerase elongation.

本数据集支持《自然化学生物学》杂志上发表的论文《通过局部生成核苷三磷酸（LAGOON）控制单分子实验中的NTP驱动反应以实现时空调控的NTPs生成》的研究发现。该论文描述了一种通过局部生成核苷三磷酸（LAGOON）来控制单分子实验中NTP驱动反应的方法，该方法提高了测量通量，并实现了单循环观测。数据集包含捕捉T4解旋酶DNA解旋、Chd1进行核小体重塑以及T7 RNA聚合酶侵入核小体的单分子FRET数据。此外，数据集还包含关于大肠杆菌RNA聚合酶延伸的声学力谱数据。