Laboratory experiments with simulated marine heatwaves to the giant kelp (Macrocystis pyrifera)@en

In this study, juvenile sporophytes were cultured using parental material from five geographically and genetically distinct populations of the giant kelp Macrocystis pyrifera from the northern and southern edges of the species' range in Aotearoa New Zealand. These sporophytes were exposed to simulated 22-day marine heatwaves at either 18°C or 22°C, or a 14°C control. The sporophytes were then kept at 14°C for a further 21 days to observe whether they showed signs of recovery. The experiment also assessed whether the long-term storage of gametophytes under red light could affect sporophyte performance; this was achieved by comparing two cultures from the same population (Wellington) that were cultured either with or without long-term storage under red light. The experiment was carried out in Wellington, New Zealand between June and July 2024. […]本研究以新西兰（奥特亚罗瓦，Aotearoa）境内巨藻（Macrocystis pyrifera）5个地理及遗传分化种群的亲本材料培养幼孢子体（juvenile sporophytes），这些种群均取自该物种自然分布范围的南北边缘地带。将这批幼孢子体分别置于18℃、22℃的模拟22天海洋热浪环境，以及14℃的对照组环境中进行处理。随后将所有幼孢子体维持在14℃环境下继续培养21天，以观测其是否出现恢复迹象。本实验同时评估了红光条件下长期保存配子体（gametophytes）是否会对孢子体生长性能产生影响，该评估通过对比来自同一种群（惠灵顿）的两组培养物完成：两组分别经过红光下长期保存与未经该处理的培养流程。本实验于2024年6月至7月在新西兰惠灵顿开展。[……]