Genome wide analysis of lower spinal cords with training after spinal cord hemisection injury

The goal of this study is to elucidate the influence of treadmill training on transcriptome of the lower lumbar spinal cord after thoracic spinal cord hemisection. mRNA profiles of spinal cords at 23 days-post injury with/without treadmill training were generated. The expression levels of 650 genes in the trained animal were increased ( > 2-fold) compared to untrained animals. Our study represents the detailed analysis of transcriptomes of spinal cord distal to the hemisected lesion after treadmill training, with biologic replicates, generated by RNA-seq technology.本研究旨在阐明胸段脊髓半切术后，跑台训练对腰段脊髓下段转录组（transcriptome）的影响。本研究生成了损伤后23天、接受与未接受跑台训练的脊髓样本的mRNA表达谱，结果显示：与未接受训练的动物相比，接受跑台训练的动物体内有650个基因的表达水平上调（幅度大于2倍）。本研究通过RNA测序（RNA-seq）技术并结合生物学重复实验，对胸段脊髓半切术后接受跑台训练的病灶远端脊髓转录组进行了详细分析。