Phenotypic data from: from buds to shoots: insights into grapevine development from the Witch’s Broom bud sport

Background Bud sports occur spontaneously in plants when new growth exhibits a distinct phenotype from the rest of the parent plant. The Witch’s Broom bud sport occurs occasionally in various grapevine (Vitis vinifera) varieties and displays a suite of developmental defects, including dwarf features and reduced fertility. While it is highly detrimental for grapevine growers, it also serves as a useful tool for studying grapevine development. We used the Witch’s Broom bud sport in grapevine to understand the developmental trajectories of the bud sports, as well as the potential genetic basis. We analyzed the phenotypes of two independent cases of the Witch’s Broom bud sport, in the Dakapo and Merlot varieties of grapevine, alongside wild type counterparts. To do so, we quantified various shoot traits, performed 3D X-ray Computed Tomography on dormant buds, and landmarked leaves from the samples. We also performed Illumina and Oxford Nanopore sequencing on the samples and called genetic ..., See the manuscript for detailed methods used for collecting and analyzing these phenotypic data., , # Phenotypic data from: From buds to shoots: Insights into grapevine development from the Witch’s Broom bud sport [https://doi.org/10.5061/dryad.5qfttdzdm](https://doi.org/10.5061/dryad.5qfttdzdm) In \"From buds to shoots: insights into grapevine development from the Witch’s Broom bud sport,\" we analyzed the phenotype and investigated the genetic basis of two independent cases of the Witch's Broom bud sport in the Dakapo and Merlot varieties of grapevine (*Vitis vinifera* L.). Here, we provide the phenotypic data from this manuscript and the link to the GitHub repository containing the code used for analyzing these data. The data provided includes i) the shoot observations used to characterize shoot traits and meristem fates, ii) the leaf scans used for comparing leaf size and shape between samples, and iii) dissecting microscope images and CT scans used to analyze internal bud morphologies. ## Description of the data and file structure The phenotypic data are divided into compressed...背景 植物在新生组织表现出与母株截然不同的表型时，会自发产生芽变（bud sport）。葡萄（Vitis vinifera）的多个品种偶发出现扫帚病芽变（Witch’s Broom bud sport），该变异会表现出一系列发育缺陷，包括矮化特征与育性降低。尽管该变异对葡萄种植者而言危害极大，但同时也可作为研究葡萄发育的有效工具。本研究以葡萄扫帚病芽变为研究对象，旨在解析芽变的发育轨迹及其潜在遗传基础。我们针对两个独立的葡萄扫帚病芽变案例开展分析，分别对应达卡波（Dakapo）与梅洛（Merlot）葡萄品种，并以其野生型植株作为对照。具体而言，我们量化了多种枝条性状，对休眠芽开展三维X射线计算机断层扫描（3D X-ray Computed Tomography），并对样本叶片进行地标标记。此外，我们还对样本进行了Illumina测序与牛津纳米孔（Oxford Nanopore）测序，并开展基因分型……详细的表型数据收集与分析方法请参见研究论文。 # 表型数据来源：《从芽到枝条：基于葡萄扫帚病芽变解析葡萄发育机制》 https://doi.org/10.5061/dryad.5qfttdzdm 在《从芽到枝条：基于葡萄扫帚病芽变解析葡萄发育机制》一文中，我们分析了达卡波与梅洛两个葡萄品种（*Vitis vinifera* L.）的两独立扫帚病芽变案例的表型，并探究了其遗传基础。本文附带提供该研究的表型数据，以及包含数据分析代码的GitHub仓库链接。本次公开的数据包括：i）用于表征枝条性状与分生组织命运的枝条观测数据；ii）用于比对不同样本间叶片大小与形态的叶片扫描图像；iii）用于分析芽内部形态的解剖显微镜图像与计算机断层扫描图像。 ## 数据与文件结构说明 本套表型数据分为压缩包……