Dicer sequencing, whole genome methylation profiling and RNA sequencing analysis in basal cell carcinoma [small RNA-seq]

Dicer sequencing was performed on the Illumina MiSeq System in a total of 16 BCC samples (8 nodular BCCs / 8 sBCCs) and mapped against the human reference genome (hg19). Dicer sequencing was performed in all 16 BCC samples. Whole genome methylation profiling performed with Infinium MethylationEPIC BeadChips as well as RNA and smallRNA sequencing were performed in 5 sBCCs with the Illumina NextSeq500 next generation sequencing system. We identified a variety of Dicer mutations that could play a role in aberrant miRNA expression in BCC. The aforementioned RNA sequences should be further evaluated in functional studies towards their possible pathogenetic role in sBCC. Overall design: Basal cell carcinoma biopsy, small RNA sequencing