Distribution of germ line mutations induced by TALENs.

To detect transmission of germ line mutations, TALEN RNA-injected G0 embryos were raised to adulthood and mated with WT partners to generate F1 offspring. gDNAs of individual 1–2 dpf F1 progeny were analyzed by HRMA for sequence alterations at targeted loci. Complete data for all G0 germ lines analyzed are presented in Tables S4, S5, S6, S7. G0 founders that transmitted mutations were further analyzed to determine the number of different mutations transmitted by each germ line and the fraction of gametes of each germ line that transmitted a mutant allele. The mean number of mutant alleles per G0 germ line was estimated based on the number of distinct HRMA melt patterns observed among the F1 progeny of each G0 founder. As analysis of the G0 germ lines was not exhaustive, the mean number of mutant alleles per G0 germ line is likely an underestimate.
*The Mean and (Range) are presented.