Methanogenic Archaeal 16S rRNA gene amplicon sequencing in Singapore fecal samples

Here, we present the analysis of the methanogenic archaea as a component of the microbiome of multi-ethnic cohort of healthy Singaporeans and show that Methanobrevibacter smithii is the most prevalent methanogen in the human gut of local subjects. Furthermore, we were able to leverage of the metagenomic sequencing results to identify fecal samples that allowed to successfully isolate a novel Methanobrevibacter strain.