Metadata record for the manuscript: Mutated clones driving leukemic transformation are already detectable at the single cell level in CD34-positive cells in the chronic phase of primary myelofibrosis

Summary

This
metadata record provides details of the data supporting the claims of the
related manuscript: “Mutated clones driving leukemic
transformation are already detectable at the single cell level in CD34-positive
cells in the chronic phase of primary myelofibrosis”.

The
related study investigated single cell genomics and transcriptomics of CD34+
cells from a primary myelofibrosis (PMF) patient who progressed to acute
myeloid Leukemia (AML) while receiving Ruxolitinib.

Data
access

Single-cell
RNA sequencing data is available via the NCBI
Gene Expression Omnibus repository with accession: https://identifiers.org/geo:GSE153319.
These scRNA sequencing data underlie Figures 3-4 and Supplementary Figures 7-8,
10-11 in the related manuscript.

Data
supporting Figure 3 and Supplementary Figure 9 are contained in the file ‘T
cells.fcs’, which is not openly available to protect patient privacy. Data
requests should be made to Prof. Manfredini.

The
patient clinical data are contained in the file ‘Patient clinical data.xlsx’,
which is also not available to protect patient privacy. Data requests should be
made to Prof. Manfredini.

All
other data supporting the related study are shared openly as part of this
metadata record. Data supporting Figures 1-2 and Supplementary Figures 1-6 are
contained in the .zip file ‘Parenti_et_al_supporting_data.zip’
and arranged in folders named according to the figures they underlie.

Data
supporting Supplementary Table 4 and Supplementary Data Files 4-6 are contained
in the file ‘Supplementary Data File 3.xlsx’, which is included with this
metadata record.

The
corresponding author is: Rossella Manfredini, PhD, Centre for Regenerative
Medicine “Stefano Ferrari”, University of Modena and Reggio Emilia, via
Gottardi n.100, 41125 Modena, Italy. E-mail: [e-mail]

Name of Institutional Review Board or ethics
committee that approved the study

The study was conducted in accordance with
the Declaration of Helsinki and with ethical approval obtained from the local
ethics committee of the Humanitas Research Hospital – Milan, Italy. (Approval
date: 28 jan 2019; approval file # 2175). The patient provided written informed
consent to take part in the study.