Constraints on human CD34+ cell fate due to lentiviral vectors can be relieved by valproic acid

We investigated whether lentiviral transduction interferes with the transcription pattern of the CD34+ cells using single-cell RNA expression analysis coupled to time-lapse microscopy. The observations show that LV transduced cells commit preferentially to common myeloid progenitor (CMP) lineage. Treatment of the cells or The cell treatment with VPA prior and during the lentiviral transduction increases the efficiency of the transduction while eliminating the preferential commitment to the CMP.