Sputum_analysis_sample_U
收藏NIAID Data Ecosystem2026-03-12 收录
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http://flowrepository.org/id/FR-FCM-Z3LX
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资源简介：
To determine whether our optimized protocol successfully characterized blood and epithelial lineage markers in a sputum sample using flow cytometry.
Conclusion:
We were able to successfully dissociate sputum into single cell suspension into live, viable cells that were stained with blood and epithelial lineage markers and analyzed by flow cytometry.
Notes:
This experiment was used as a proof of concept of our protocol for a methods manuscript. No treatments or experimental conditions were applied to the samples. FlowCheck was performed to check the fluidics and optics. FlowSet was performed to standardize the machine for the sputum specific settings. The presence of alveolar macrophages was verified to determine the sample derives from the lung.
创建时间：
2021-06-01
