Human HFF cells large rearrangements detection after CRISPR-Cas9 nuclease (Fig. 5e)

ABRAXAS2 or SORSC1 gene editing by CRISPR-Cas9 nuclease in heterozygous HFF KO P53 for UROS inactivation. Fluorescent positive cell sorting for UROS and large rearrangements confirmation in chromosome 10q. Two-condition experiment, florescent-positive clones HFF KO P53 edited vs fluorescent-negative HFF KO P53 edited bulk after ABRAXAS2 or SORSC1 gene editsing.