Stability of selected CV-A13.67900/MAD4 and CV-B4/MAD4 recombinants upon serial passaging.

a The recombinant group is indicated in brackets. The name of a recombinant includes the type (e.g. CV-A13.67900/MAD4) and class (A) followed by the laboratory number (64).b The 5’ UTR domain in which the recombination site is located is indicated in brackets. For example, (dIII-dIV) designates the linker sequence between domains dIII and dIV.c In these particular homologous recombinants (which contain neither an insertion nor a deletion) a few partner nt were replaced with different nt at the junction (sequence indicated).d The length of insertions (+) and deletions (-) is given. For insertions, the duplicated IRES domains are indicated in square brackets. Homologous recombinant sites are shown (H).e Number of passages. The status of the resulting recombinant is indicated following passaging. Homologous: loss of insertion. Parental: recombination site unchanged, conservation of the parental virus sequences. Mixture: mixture of parental and homologous recombinants.Stability of selected CV-A13.67900/MAD4 and CV-B4/MAD4 recombinants upon serial passaging.