Aquafin CRC Salmon Project - Process Study 3- Microheterotrophic Grazing Experiment - North West Bay - October 2006

The Aquafin CRC Salmon Project is being undertaken by CSIRO Marine and Atmospheric Research (CMAR) and Tasmanian Aquaculture and Fisheries Institute (TAFI). Process Study 2 was undertaken between 3-5 October 2006 in the North West Bay. Prior to the three microheterotroph grazing experiments, a CTD (Seacat 4550) cast was performed at site NWB2 to determine the depth of the fluorescence maximum. Water for the three grazing experiments was collected simultaneously from a depth of 15m. For the first experiment, the sample water was filtered through a 200µm mesh. For the second experiment, no screening was used for the sample water. In the third experiment approximately 100 ml of water containing microzooplankton, which had been previously screened through a 1mm-mesh, was added to the sample water. Samples were incubated for 24 hours with subsamples collected at times T0 and T24 for phytoplankton and microheterotroph counts, nutrient analysis and pigment concentration and composition. Grazing and growth rates were calculated.

Aquafin CRC三文鱼项目由澳大利亚联邦科学与工业研究组织海洋与大气研究所（CSIRO Marine and Atmospheric Research，CMAR）与塔斯马尼亚水产与渔业研究所（Tasmanian Aquaculture and Fisheries Institute，TAFI）联合实施。过程研究2于2006年10月3日至5日在西北湾开展。在三项微异养生物摄食实验开展前，研究人员于NWB2站位使用CTD（Seacat 4550）开展温盐深剖面测量，以确定荧光最大值的出现深度。三项摄食实验所用的水体均同步从15米水深处采集。第一项实验中，样品水体经200μm筛网过滤；第二项实验中，未对样品水体进行筛滤处理；第三项实验中，向样品水体中加入约100ml预先经1mm筛网过滤的含微型浮游动物的水样。所有样品均培育24小时，并于T0和T24时刻采集子样品，用于浮游植物与微异养生物计数、营养盐分析以及色素浓度与组成测定。最终计算得到摄食率与生长率。