The sequence and de novo assembly of Larimichthys crocea genome using PacBio and Hi-C technologies

Larimichthys crocea
is an endemic marine fish in
East Asia that belongs to Sciaenidae
in Perciformes. L. crocea now
has been recognized as an "iconic" marine fish species in China not
only because it is traditional food fish for people in China, but also because
it is the most recognized overfishing victim and still providing high value
fish products via large scale mariculture. Here we report a chromosome-level
reference genome of L. crocea generated
employing the PacBio single molecule sequencing technique (SMRT) and
High-through chromosome conformation capture (Hi-C) technologies. The genome
sequences were assembled into 1,591 contigs with a total length of 723.86 Mb
and a contig N50 length of 2.83 Mb. After chromosome-level scaffolding, 24
scaffolds were constructed with a total length of 668.67 Mb (92.48% of the
total length of all contigs). Genome annotation identified 23,657
protein-coding genes, 2,517 ncRNAs. The high accurate, chromosome-level reference
genome of L. crocea provides the essential
genome resource to develop genome-scale selective breeding and restocking strategies
of L. crocea.