RNA-seq analysis of hearts from control and cardiomyocyte-specific PRMT1-deficient mice

We found that cardiomyocyte-specific PRMT1-deficient (PRMT1-cKO) mice showed dilated cardiomyopathy and aberrant cardiac alternative splicing. To identify novel cardiac splicing events, we performed a comprehensive analysis of gene expression changes in hearts of wildtype (WT) and PRMT1-cKO mice using RNA sequencing (RNA-Seq). To investigate differentially expressed genes (false discovery rate (FDR) p<0.05, fold change >2) between control and PRMT1-cKO mice, we performed pairwise comparisons of RNA-Seq data using the CLC Genomics Workbench software. Examination of mRNA profile and alternative splicing in control and PRMT1-cKO mice (n=2).我们发现，心肌细胞特异性PRMT1敲除（PRMT1-cKO）小鼠可表现出扩张型心肌病与异常心脏可变剪接表型。为鉴定新型心脏剪接事件，我们采用RNA测序（RNA-Seq）技术，对野生型（WT）与PRMT1-cKO小鼠的心脏组织开展了全面的基因表达变化分析。为探究对照组与PRMT1-cKO小鼠间的差异表达基因（错误发现率（FDR）p<0.05，倍数变化>2），我们使用CLC基因组工作台（CLC Genomics Workbench）软件对RNA-Seq数据进行了两两比较分析。本研究对对照组与PRMT1-cKO小鼠（n=2）的mRNA表达谱及可变剪接情况进行了检测。