RNA-seq gene expression profiling of livers from wild-type male and female mice, and from mice with a Double Knockout (DKO) of Ezh1 and Ezh2.

PolyA-selected RNA isolated from livers of adult male and female mice with a combined loss of EZH1 and EZH2 in hepatocytes was analyzed by RNA-seq. The DKO mouse model used in these analyses is a global deletion of Ezh1 and hepatocyte-specific deletion of Ezh2, and is described in Bae WK, Kang K, Yu JH, Yoo KH, Factor VM, Kaji K, Matter M, Thorgeirsson S, Hennighausen L. FASEB J. 2015 May;29(5):1653-62. doi: 10.1096/fj.14-261537. PMID: 25477280 Liver RNA, polyA-selected, was isolated from 7 week old male and female DKO mice (Ezh1-/-, Ezh2-f/f_Alb-Cre) and their floxed (f) controls. RNA samples from n=3 mouse livers were pooled to make each of 3 biological replicate libraries per sex and genotype. AC, Albumin-Cre