Revealing the Immune Response of Sitona callosus to Beauveria bassiana Infection through Integrative Analyses of Transcriptomics and Metabolomics

The legume pest Sitona callosus poses a significant threat to alfalfa growth. Due to its unique life cycle, Beauveria bassiana has emerged as an effective fungus for controlling S. callosus . We conducted a comprehensive analysis of the transcriptome and metabolome of S. callosus infected by B. bassiana. Differential expression of antifungal genes, including heat shock genes, cytochrome P450 (CYP450) genes, cathepsin proteases, and C-type lectin were identified in S. callosus. Pathway analysis revealed associations between immune genes and metabolites involved in autophagy-animal, glucagon signaling pathway, and glycerophospholipid metabolism. These findings provide valuable insights for enhancing the control efficacy of Beauveria bassiana on Sitona callosus. In order to determine the immune response of Sitona callosus infected by Beauveria bassiana. Transcriptomic tests were performed on Sitona callosus after infection with Beauversiana bassiana and the uninfected one.