Effect of nuclear pore complex (NPC) blockage by WGA treatment on zygotic genome activation (ZGA) during zebrafish early development

To investigate the function of nuclear pore complex (NPC) in the regulation of zygotic genome activation (ZGA), we microinjected medium dosage of WGA in zebrafish embryos at 1-cell stage to block NPC function. We then performed gene expression profiling analysis using data obtained from RNA-seq of control or WGA treated embryo at comparable developmental time point (4.3 hours post fertilization (hpf) ) or develomental stage (dome). Overall design: Comparative gene expression profiling analysis of RNA-seq data for zebrafish control embryo at 4.3 hpf of dome stage and WGA treated embryos at 4.3 hpf (developmental time matched) or 5 hpf (dome-like stage).