Assembled unigens for Curcuma kwangsiensis

<i>C. kwangsiensis</i> RNA was used to construct cDNA libraries with fragment a length of 200 bp. Then, paired-end sequencing was performed using an Illumina HiSeq^TM 2500 following the manufacturer’s instructions (Illumina, San Diego, CA). The quality of the raw reads was assessed using SeqQC-V2.2 software, and high-quality reads (Q &lt; 20) were assembled using the Trinity software with default parameters. Only these high-quality, filtered reads were utilized for further analysis. The assembled sequences were compared against the nonredundant protein database (nr) using BLASTX with a threshold E-value of 10^-6.