RNA-Seq analysis was performed with total RNA isolated from mature pollen of Col-0 and sks13 mutant

To establish the regulatory roles of SKS13 in pollen development , we identified the regulated genes whose transcriptional activities are associated with relative abundance of SKS13 transcripts by transcriptomic analysis. Down-regulated genes in sks13-1 were mainly responded to JA, cell wall synthesis and oxygen levels. Up-regulated genes in sks13-1 were predicted to be associated with pollen tube growth.