Burkholderia pseudomallei promoter trap _ Raw sequence reads

Burkholderia pseudomallei, the causative agent of melioidosis, can survive and replicate in macrophages; however, little is known about B. pseudomallei genes that are induced during macrophage infection. Here, we constructed a promoter library of B. pseudomallei using a gfp-promoterless plasmid and then introduced this into the closely-related bacterium B. thailandensis to allow screening of infected macrophages by cell sorting under biosafety level-2 conditions. The library was depleted of fusions that were active during growth on laboratory medium. After selection of clones activated during infection of macrophages, Illumina sequencing of PCR amplified inserts identified B. pseudomallei promoters that were induced during infection.