Heterologous Expression of Fluostatin Gene Cluster Leads to a Bioactive Heterodimer
收藏Figshare2016-02-12 更新2026-04-29 收录
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https://figshare.com/articles/dataset/Heterologous_Expression_of_Fluostatin_Gene_Cluster_Leads_to_a_Bioactive_Heterodimer/2112352
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The biosynthesis gene cluster (fls) for atypical angucycline fluostatins was identified from the marine derived Micromonospora rosaria SCSIO N160 and was confirmed by gene knockouts and the biochemical characterization of a bifunctional oxygenase FlsO2. The absolute configuration of the key biosynthetic intermediate prejadomycin was determined for the first time by Cu Kα X-ray analysis. Heterologous expression of the intact fls-gene cluster in Streptomyces coelicolor YF11 in the presence of 3% sea salts led to the isolation of two new compounds: fluostatin L (1) and difluostatin A (2). Difluostatin A (2), an unusual heterodimer, exhibited antibacterial activities.
创建时间：
2016-02-12
