Wheat roots and soil DNA extraction under two CO2 levels Raw sequence reads. Wheat roots and soil DNA extraction under two CO2 levels

During plant growth, from seed germination until maturity, roots release specificcompounds to the surrounding soil. The composition of root deposits depends on planttype and age, leading to altering structure and function of root microbiome reacting tothese compounds. Atmospheric CO2 levels had been increasing for the last 150 years,stimulating growth and photosynthesis in C3 plants (e.g. wheat), increasing rootdeposition.In the current study, effect of elevated CO2 on wheat-root microbiome composition andplant development was studied, with emphasis on denitrifying communities.Wheat plants were grown in greenhouse equipped with two CO2 chambers: 1) ambientCO2 of 400 ppm (aCO2); 2) elevated CO2 of 850 ppm (eCO2). Soil, roots and leaveswere sampled during six weeks of growth and chemical and microbial parameters weremeasured. Total bacterial community was quantified using qPCR with universal 16SRNA gene primers, while denitrifying genes (e.g. nirK, nirS, nosZ) were measured usingspecific primers constructed based on metagenome sequencing. In addition, total andN 2 O reducing bacterial community in soil and root were sequenced.