5-fluoro-tryptophan USP5 Zf-UBD Growth & Purification for 19F NMR Screening
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The expression and purification of USP5171-290 in media supplemented with 5-fluoro-tryptophan. Mass spectrometry, differential scanning fluorimetry and fluorescence polarization assays were used to verify label incorporation, protein folding and ubiquitin peptide binding respectively. Preliminary 19F NMR experiments were completed with the purified protein to determine if there are perturbations in one of the 19F resonances with a ubiquitin peptide, LRLRGG.
创建时间：
2024-08-02
