Hans Ris (2010) CIL:7503, gallus gallus, erythrocyte. CIL. Dataset

A drop of a preparation of chicken erythrocyte nuclei was exposed to low ionic strength and centrifuged onto a formvar-carbon film, fixed with paraformaldehyde and negatively stained with sodium phosphotungstate. Images were recorded at 40KX and accelerating voltage of 1MeV at the University of Wisconsin HVEM facility.This image was taken with a specimen tilt of 55 degrees. A grouped image of the same field at a 45 degree tilt provides an oblique stereo view of the tangle of chromatin fibers in the dispersed nucleus.

一滴制备的鸡红细胞核悬浮液在低离子强度条件下进行处理，并通过离心作用沉积于碳膜上。随后，使用多聚甲醛进行固定，并以钨磷酸钠进行负染色。图像记录于威斯康星大学HVEM设施，采用40KX的放大倍数和1MeV的加速电压。本图像拍摄时样本倾斜角度为55度。同一视野下，以45度倾斜拍摄的成组图像提供了对分散核中染色质纤维错综复杂结构的斜视立体观。